FIGURE 2.
Facilitatory effects of astrocyte inhibition by fluorocitric acid (FCA) on CeLC neuronal properties in a neuropathic model. Neurons recorded from FCA-treated slices (100 μM, 1 h) showed increased neuronal excitability (A,B) induced by depolarizing current injections, while Ba2+ pretreatment had no effects compared to the control (ACSF) group (*, p < 0.05, two-way ANOVA with Bonferroni post hoc tests). Symbols show means ± SEM. ***, p < 0.001, two-way ANOVA. In the same neurons, FCA had no effects on rheobase (C) or AP threshold (D,E), but it decreased the depolarizing voltage sag (F,G) without affecting the I-V relationship (H,I) compared to the control (ACSF) treated cells. Bar histograms show means ± SEM. *, p < 0.05, unpaired student t-tests. Symbols show means ± SEM, two-way ANOVA. ACSF, n = 7; FCA, n = 8.