Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 21;15:1368634. doi: 10.3389/fphar.2024.1368634

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2024 Mazzitelli, Ponomareva, Presto, John and Neugebauer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Validation of astrocyte inhibition by FCA in CeA in neuropathic rats. Immunohistochemical analysis showed decreased percentage of astrocytic GFAP positive area, but not mean of positive signal, in the CeC (A,B) and CeL (C,D) after FCA (100 μM, 1 μL) injection into the CeA of SNL (4 weeks) rats compared to vehicle (Veh) group, consistent with astrocyte inhibition in the targeted area. NeuN staining showed no significant differences between the two groups (E–H), confirming the glia-specific effect of the pharmacological approach. Bar histograms show mean ± SEM. **, ***p < 0.01, 0.001 compared to vehicle, unpaired student t-tests. Veh, n = 5; FCA, n = 5 (I) Representative images of GFAP (+) staining in brain sections from Veh (left) and FCA (right) injected neuropathic rats. Scale bar = 200 μm.