Fig. 3.

Internalization of anti-GD2 antibody by human neuroblastoma cell lines is inversely correlated with sensitivity to neutrophil-mediated ADCC in vitro. a Scatter plot of mean GD2 expression (ABC) for cell lines shown in Fig. 1a and corresponding 14G2a-pHrodo internalization 24 h mean normalized AUC (r = 0.32). b Scatter plot of 24 h anti-GD2 internalization AUC (normalized mean) versus average cytotoxicity (% at 6 h) to neutrophil-mediated ADCC (measured on an Incucyte) for the 11 cell lines with both data points assessed (SMS-KANR, SMS-KAN, SMS-KCN, LAN5, CHLA15, CHLA255, CHLA20, CHLA90s, SMS-KCNR, LAN1) (r = −0.41). c Mean 24 h normalized AUC of 14G2a internalization for all tested NBL cell lines sorted by disease status at time of cell line initiation (Dx = diagnostic, PD = progressive Disease; left panel) or MYCN status (NA = non-amplified, Amp = amplified; right panel) (ns = p value non-significant)