Fig. 4.

Treatment with endocytosis inhibitors decreases anti-GD2 antibody internalization. a Mean (± SD) 24 h AUC (expressed as % of control) of 14G2a internalization measured by Incucyte for Lan1GFP cells, a high rate antibody-internalizing cell line, following a 30-min pretreatment with endocytosis inhibitors at noted concentrations or media-only control, (*p < 0.05, **p < 0.01). b Representative red fluorescent images of 14G2a internalization by Lan1 subjected to each inhibitor treatment as shown in (A) at 6, 12, and 24 h. c Average (± SD) GD2 surface expression of Lan1 cells by flow cytometry quantified by BD Quantibrite beads following a 30 min treatment with endocytosis inhibitors (EIPA, MBCD, chlorpromazine, Pitstop2) or control (average of 3 replicates; p > 0.05 for all)