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Detection of vRNA-2 with strand-specific probes. Shown is an autoradiogram of a Northern blot where vRNA was separated on a formaldehyde gel and probed with strand-specific riboprobes. Lanes: 1, vRNA hybridized with a riboprobe generated in the nt 92576-to-93513 direction (top strand); 2, vRNA hybridized with a riboprobe generated in the nt 93513-to-92576 direction (bottom strand). The arrow in lane 2 indicates the broad band detected when probes generated from the top strand were used.
