Fig. 1.

miR-34a upregulated B7-H3 in CRC. a miR-34a-mediated regulation of genes in HCT-116 cells. HCT-116 cells were transfected with 50 nM miR-34a mimics for 48 h. Total RNAs were sequenced by Illumina HiSeqTM 2500. b miR-34a mimics significantly increased B7-H3 mRNA expression in HCT-116 cells in a dose-dependent manner. c miR-34a mimics significantly increased B7-H3 protein expression in HCT-116 cells in a dose-dependent manner. B7-H3 protein expression was measured by WB method. d miR-34a mimics (50 nM) significantly increased B7-H3 protein expression in HCT-8 cells. e The expression of miR-34a in the CRC tissues was significantly higher than the normal tissues. f B7-H3 protein expression was positively correlated with miR-34a in the CRC tissues. Each experiment was performed in duplicate. Data represent mean ± SD. Significance was assessed by t-test. ***p < 0.001; **p < 0.01; *p < 0.05