Fig. 4.

Identification of the epitope of the Q1 TIL clone. a Q1 TIL clone reactivity to serially truncated peptides. Serially truncated mutant peptides encoded by mutant TNS1 were examined for reactivity to the Q1 TIL clone. Each peptide was pulsed onto 293 T-C*03:03 cells at different concentrations, and their recognition by the Q1 TIL clone was assessed by an IFNγ ELISPOT assay. IFNγ spots are shown as mean ± SEM (n = 3). b The Q1 TIL clone recognizes the mutant peptide, but not the wild-type peptide. Two peptides, 9Y and 9S derived from mutated and non-mutated TNS1 gene, respectively, were examined for reactivity to the Q1 TIL clone. Each peptide was pulsed onto 293 T-C*03:03 cells at different concentrations, and their recognition by the Q1 TIL clone was assessed by an IFNγ ELISPOT assay. IFNγ spots are shown as mean ± SEM (n = 3)