Fig. 5.

Immunological and genetic heterogeneity of the primary tumor. a The Q1 TIL clone recognizes primary RCC cells derived from the red tumor, but not RCC cells derived from the yellow tumor. Q1 TIL clone reactivity for primary RCC cells derived from the red and yellow tumors. To confirm specificity, an anti-HLA-BC antibody (B1.23.2) was used. IFNγ spots are shown as mean ± SEM (n = 3). b TNS1 gene mutation is detected only in RCC cells derived from the red tumor. DNA sequencing was performed using genomic DNA derived from 1226 RCC cells, red tumor, yellow tumor, normal kidney, and PBMCs. The 1309Y mutation was detected only in 1226 RCC cells and the red tumor