Fig. 1.

oAD-IL7 successfully replicate in glioma. a Schematic representation of genome of the oncolytic viruses used in this study. b Monolayers of glioblastoma cell lines were infected by oAD-IL7 at the MOI of 10. Cells were imaged with a fluorescence microscope 24, 48, and 72 h after infection. Scale bar, 500 μm. c Cells were collected 72 h after infection for plaque assay. Each number of the virus tilter were calculated on average of three independent wells. d Oncolytic virus-induced GBM apoptosis was confirmed by western blot. Scale bar, 100 μm. e Cells were stained with IL7 antibody 72 h after infection and imaged with a fluorescence microscope. Green fluorescence represents the oAD-IL7 infection; red fluorescence represents the IL7 distribution; blue fluorescence represents the cell nuclei stained with DAPI. Scale bar, 50 nm. f Supernatants were collected for IL7 quantification. g The median tumor volume of the xenograft models treated by PBS, oAD, and oAD-IL7 was recorded. h Survival analysis of the three group of the xenograft models. *P < 0.05; **P < 0.01; ***P < 0.001 (two-tailed paired t test)