Fig. 2.

B7H3-CAR-T exerts selective cytotoxicity to B7H3-positive GBM cells. a Schematic representation of B7H3-CAR. b Transduction efficiency of B7H3-CAR-T was calculated by flow cytometry 48 h after lentivirus infection. c Different GBM cell lines were stained with anti-B7H3 to calculate the levels of B7H3 expression. d B7H3-CAR-T and untransduced T cells were collected and stained with CD25 and CD69 after coculturing with U87 for 48 h (E:T = 5:1). e Expression of the IL7R (CD127) on the modified B7H3-CAR-T cells. f Supernatants were obtained after 48 h coculturing (E:T = 5:1), and the concentration of TNF-α and IFN-γ were analyzed by Elisa assay. g ⁵¹Cr-release assays were used to evaluate the lysis of GBM cells after treated with T cells at an E:T rate of 5:1 or 1:1. Representative results from three independent experiments are shown. *P < 0.05; P < 0.01; ***P < 0.001 (two-tailed paired t test)