Fig. 3.
oAD-IL7 enhanced the survival and efficacy of B7H3-CAR-T. a 1 × 10 B7H3-CAR-T or untransduced T cells underwent serial coculturing with 2 × 105 oncolytic virus-infected U87 cells for a total of 7 days. The GBM cells were refreshed on the 4th day of the serial coculture. b Cumulations of T cells expansion. c For proliferation analysis, T cells were stained with CytoTell Blue before serial coculture and collected for flow cytometry at the end of day 3 and day 7 to monitor the results of division. d For survival analysis, T cells were collected and stained with Annexin V and 7-AAD. e 51Cr-release assays were used to evaluate the lysis of GBM during the second round of coculture at the end of day 7. Data represent the mean ± SD of triplicate wells. *P < 0.05; **P < 0.01 (two-tailed paired t test)