Fig. 1.

mAb04-MICA inhibited proliferation, promoted apoptosis, and altered cell cycle progression of tumor cells. A, mAb04-MICA bound to human tumor cell lines: AGS, BGC-823, and normal cell lines:HL-7702 by flow cytometry. B, mAb04-MICA, mAb04, and ramucirumab inhibited the proliferation of cell lines by MTT assay. C, BGC-823 cells and H157 cells were incubated with 200 nM mAb04 or mAb04-MICA at 37 °C for 72 h and analyzed by flow cytometry after staining with Annexin V-FITC and PI. Quantitative analysis is shown on the right. D, Representative images of cell cycle analysis by flow cytometry in BGC-823 cells and H157 cells that were incubated with mAb04 or mAb04-MICA. Quantitative analysis is shown on the right. E, western blot analysis for expressions of p-VEGFR2/VEGFR2, p-AKT/AKT, p-ERK/ERK, and p-P38/P38 by BGC-823 cells treated with VEGF with or without mAb-MICA. F, Expression of Bcl-2 family members Bax, Bak, Bcl-2, and Bcl-xl was examined by western blot. (Data are presented as the mean ± SD, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001)