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. 1998 Sep;72(9):7005–7011. doi: 10.1128/jvi.72.9.7005-7011.1998

FIG. 1.

FIG. 1

Schematic representation of the DNA sequence arrangements of the wild-type and recombinant viruses used in this study. Line 1, schematic representation of the wild-type HSV-1 genome. The genome consists of two covalently linked components, L and S, each consisting of unique sequences (UL and US) flanked by inverted repeats. The arrangement shown is the IS isoform in which the S component is inverted relative to the prototypic orientation of the L component. The inverted repeat sequences designated ab and b′a′ flanking the UL sequence are 9 kb in size, whereas the repeat sequences ac and c′a′ flanking the US sequence are each 6.3 kb in size. Line 2, expansion of specific domains of the genome showing gene arrangements within the expanded region. Line 4, schematic representation of one of two γ134.5 coding domains in recombinant R3616 in which the sequences between the BstEII and StuI restriction endonuclease sites had been deleted. Line 6, representation of a portion of the genome of the recombinant R7023 shown in the IS arrangement. Line 7, schematic representation of the wild-type UL23 gene encoding thymidine kinase and key restriction endonuclease sites present in R7023. The domain encoding the genes US8 through US12 as well as the reiterated sequences a′ and ac and the portion of the b′ sequence encoding one of the copies of the γ134.5, ORF O, and ORF P genes are absent. R7023 was the parent of R5103 schematically represented in line 9. In R5103, the coding domain between the BstEII and StuI restriction sites in the remaining copy of the γ134.5 gene was replaced with the E. coli lacZ gene represented in line 10. Line 13, schematic representation of the recombinant virus R5104 constructed by homologous recombination between plasmid pRB4999 and R5103 DNA. In the resulting recombinant, R5104 (line 14), the UL23 gene was disrupted by the insertion of US10 and US11 driven by the α47 promoter. Lines 3, 5, 8, 11, 12, and 15 represent the predicted bands produced by restriction endonuclease digestion of viral DNAs and are shown as reference for the bands shown in Fig. 2. Abbreviations: B, BamHI; N, NcoI; Bs, BstEII; St, StuI; Sc, SacI; Bg, BglII.