FIG. 6.
(A) Autoradiographic image of electrophoretically separated purified eIF-2 samples after reaction in the presence of [γ32P]ATP with S10 fractions from cells mock-infected or infected with HSV-1(F), R7023, R3616, R5103, or R5104. The arrows indicate the position of the α subunit of eIF-2 and the Mr-39,000 protein unrelated to eIF-2. (B) Autoradiographic image of electrophoretically separated proteins contained in the eIF-2 kinase reactions carried out as described above. The samples were electrophoretically separated and run on a longer gel to increase the separation of the Mr-39,000 phosphoprotein from eIF-2α. The arrows indicate the position of the α subunit of eIF-2 and the unrelated Mr-39,000 phosphoprotein present in the purified eIF-2 samples.