Fig. 3.

Immune response monitoring. Detection of specific immune responses. (a + b) Intracellular cytokine staining (ICS) performed on mononuclear cells from time points before and after vaccination, stimulated with either an irrelevant control peptide (red) or a pool of peptides from the vaccine (blue). Gated on (a) CD4 or (b) CD8 cells positive for CD107a, Interferon-gamma (IFNy) or Tumor Necrosis Factor-alpha (TNFa). (c) ELISpot comparing T cell reactivity before (red) and after (blue) vaccination. Experiment performed on PBMCs pre-stimulated with the individual vaccine peptides, an irrelevant peptide control, no peptide, or a cocktail of viral peptides (CEFT). Spot forming units (SFU) per 100.000 cells were normalized by subtracting the background signal from wells containing the irrelevant control peptide. ELISpot experiments were performed in triplicates. ELISpot for P4 is showed separately in Supplementary Fig. 3