Fig. 7.

Screening and culture of positive clone cells expressing both the lentiviruses of sh-CXCL2 and sh-CXCR2 which can reverse the phenotype of EMT and rescue the signalling pathway of JAK1/STAT3. a The lentiviruses of sh-CXCL2 and sh-CXCR2 were co-transfected into TSCCa cells. b Flow cytometry was used to screen out the positive cells that expression both lentiviruses of sh-CXCL2 and sh-CXCR2. c The clone of positive cells were screened out and cultured by limited dilution method. d Western blot analysis after inactivation of the CXCL2/CXCR2 signalling axis in the P. gingivalis-infected TME of OSCC, the activity of the JAK1/STAT3 signalling pathway was decreased, and the EMT process was reversed. (*p < 0.05, **p < 0.01, and ***p < 0.001; one‐way analysis of variance)