FIG. 4.

(A) Southern analysis of an AAV2-Rluc vector stock produced by TtetA2Rluc c.49 cells and fractionated on a CsCl gradient. The AAV2-Rluc ssDNA present in each gradient fraction was released from vector particles, separated on a 1.2% alkaline agarose gel, and probed for luciferase sequences. A 3.85-kb BamHI fragment containing the luciferase expression cassette present in AAV2-Rluc served as a standard. The expected size of the vector DNA is 4.17 kb. TtetA2Rluc c.37 gave very similar results (not shown). (B) The density and percentage of the total amount of AAV2-Rluc genomes present in each gradient fraction were plotted based on quantification of the blot in panel A. The fraction containing Ad is indicated.