Fig. 3.

B7-H3 suppresses the expression of ULBP2 in colon cancer cells in vitro and in vivo. a, b The mRNA expression of NKG2DLs, including ULBP1, ULBP2, ULBP3, ULBP4, ULBP5, MICA, and MICB, in B7-H3-overexpressing (a) or knockdown (b) HCT116 and RKO cells was analyzed by RT-qPCR. n = 3 (c, d) The protein expression of ULBP2 in B7-H3-overexpressing (c) or knockdown (d) HCT116 and RKO cells was analyzed by western blot assay. β-Actin served as a loading control. e, f The protein expression of ULBP2 in B7-H3-overexpressing (e) or knockdown (f) HCT116 and RKO cells was analyzed by flow cytometry assay. n = 3 (g) The expression of B7-H3, ULBP2 and infiltrating γδT cells in B7-H3-overexpressing HCT116 tumors in SCID mice following Vδ2 T cell treatment was analyzed by immunohistochemistry or immunofluorescence assay. n = 5 mice per group. Values are expressed as the means ± SEM. **p < 0.01, and ***p < 0.001