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. 2021 Nov 1;71(7):1569–1582. doi: 10.1007/s00262-021-03096-y

Table 1.

Primers used in this study for HLA-A genotyping

Primers Sequence (5′-3′) Anneal site Product
A1 GAAACSGCCTCTGYGGGGAGAAGCAA Intron l: 21–26 985 bp
A3 TGTTGGTCCCAATTGTCTCCCCTC Intron 3: 66–89
A2F AGCCGCGCCKGGASGAGGGTC Exon 2- intron 2: 99–119 270 bp
A2R GGCCCGTCCGTGGGGGATGAG Exon 2- intron 2: 37–57
A3F GTTTCATTTTGRTTKAGGCCA Exon 3- intron 3: 150–171 276 bp
A3R TGTGGGAGGCCAGCCCGGGAGA Exon 3- intron 3: 41–66