Table 1.
Primers | Sequence (5′-3′) | Anneal site | Product |
---|---|---|---|
A1 | GAAACSGCCTCTGYGGGGAGAAGCAA | Intron l: 21–26 | 985 bp |
A3 | TGTTGGTCCCAATTGTCTCCCCTC | Intron 3: 66–89 | |
A2F | AGCCGCGCCKGGASGAGGGTC | Exon 2- intron 2: 99–119 | 270 bp |
A2R | GGCCCGTCCGTGGGGGATGAG | Exon 2- intron 2: 37–57 | |
A3F | GTTTCATTTTGRTTKAGGCCA | Exon 3- intron 3: 150–171 | 276 bp |
A3R | TGTGGGAGGCCAGCCCGGGAGA | Exon 3- intron 3: 41–66 |