Fig. 5.
COM902 enhances human T cell function. a COM902 increases IL-2 signaling. Representative data (n ≥ 2) shows the RLU (mean ± SD) of the luciferase signal from a 6 h co-culture of Jurkat IL-2-RE luciferase human TIGIT cells and CHO-K1 human PVR cells. A 20 point, 1.5-fold dilution series starting at 133 nM was used for each antibody. b The expression of PD-1, PVRIG and TIGIT on CMV-reactive CD8+ T cells (top row, n = 3 donors), and of PVR, PVRL2, PD-L1, and HLA-A2 on Mel-624-pp65 was assessed. White histograms represent the isotype control staining, and gray represents the expression of the target of interest. c CMV-reactive CD8+ T cells were co-cultured with Mel-624-pp65 cells for 18 h in the presence of 10 µg/mL COM902 or hIgG4 isotype control antibody. Percent change in IFN-γ for each condition relative to isotype control is depicted by the number above each bar. The bar graphs show the average ± SD. Data were analyzed by paired Student t-test; *p < 0.05; **p < 0.01; ***p < 0.001. Data shown is representative of n ≥ 3 experiments (n = 3 donors). d CMV-reactive CD8+ T cells were co-cultured with CMV peptide-pulsed Mel-624 cells engineered to express hPVR, hPVRL2, and luciferase in the presence of COM902 in two-fold dose-titration range from 6.6–0.006 nM. Percent specific cytotoxicity was calculated by