Fig. 2.

Low UBR5 expression is predictive of unfavorable clinicopathological characteristics and poor postoperative prognosis of ccRCC patients. a Representative images of H&E and IHC staining of UBR5 in ccRCC specimens from the training cohort are presented (scale bar = 20 µm). b A time-dependent receiver operating characteristics (ROC) analysis was used to determine the optimum cutoff value of UBR5 to predict 5-year overall survival (OS) in the training cohort (according to a 3:2 ratio). c–f Kaplan–Meier curves for OS and progression-free survival (PFS) of ccRCC patients were analyzed based on the UBR5 expression in the randomized training cohort (c, d) and the validation cohort (e, f) (3:2 ratio). g A time-dependent ROC analysis was used to calculate the optimum cutoff value of UBR5 to predict 5-year OS in the training cohort (1:1 ratio). h–k Kaplan–Meier curves for OS and PFS of ccRCC patients were analyzed according to UBR5 expression in the randomized training cohort (h, i) and validation cohort (j, k) according to a 1:1 ratio. l A time-dependent ROC analysis was used to examine the optimum cutoff value of UBR5 to predict 5-year OS in the combined cohort. m–n Kaplan–Meier curves for OS and PFS of ccRCC patients were analyzed according to UBR5 expression in the combined cohort. All p values are defined as: *p < 0.05; **p < 0.01 and ***p < 0.001