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Peptide identified from Pepsin-ISPH4h-1P F1 fraction. A the mass spectrum of a peptide, EKPQQQSSRRGS, identified by LC–MS–MS. Effects of the synthetic peptides, EKPQQQSSRRGS (SP1) and VVQGKGAIGFAFP (SP2), on positive fluorescent level in macrophages (B), and M1 (C) phenotype polarization in mouse spleen in vivo. Bars represent mean ± standard deviation (SD; n = 3). Mean with different letters are significantly different (p < 0.05) by Duncan’s multiple range test
