Fig. 5.

CD27 signal enhances T2-CAR T cells survival. a The proportions of CAR T cells in CD8⁺ T cells in tumor tissue and spleen (b) at 7 d after CAR T cell infusion in MC38-Trop2⁺ tumor-bearing mice (mean ± SEM; n = 4 for each group). c T2-mCAR T cells were co-cultured with MC38-Trop2⁺ cells. Apoptosis levels in mCAR T cells were analyzed using Annexin V/7-AAD dye‐staining at 12 h post-stimulation. d, e Early and late apoptosis levels of co-cultured CAR-T cells were detected at different time periods (mean ± SEM; n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001