Fig. 1.

Generation of anti-human mesothelin CAR-T cells producing human IL-7 and CCL19. a Schematic representation of Conv. CAR and 7 × 19 CAR against human mesothelin. LS; leader sequence, TM; transmembrane domain. b Human PBMCs transduced with Conv. CAR or 7 × 19 CAR were stained with recombinant mesothelin protein to detect CAR expression, along with anti-CD8 Ab. UTD T cells were examined, as a control. The percentage of cells in each quadrant are indicated. c The culture supernatants from Conv. CAR-T and 7 × 19 CAR-T cells were harvested 4 days after gene transduction, and the concentrations of IL-7 and CCL19 were measured using ELISA. As a control, the culture supernatant from UTD T cells at the same time point were examined. Open circle represents the value of an individual sample. Data are shown as mean ± standard deviation (SD) of triplicate samples