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. 2021 Nov 26;8(1):115. doi: 10.1186/s40643-021-00471-6

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© The Author(s) 2021

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Fig. 2 — Secretory expression of HTHHly. a Plasmid pET-HTHHly. b Verification of the expression strain harboring the two plasmids. Lane 1: plasmid pET-HTHHly; Lane 2: plasmid pBAD-BD; Lane 3: plasmid extracted from BL21(DE3)-pET-HTHHly + pBAD-BD. c Secretory expression of HTHHly. W whole-cell lysate, S supernatant. The arrow indicates the target protein HTHHly