Table 3.
Summary of the plasmids, DNA constructs, and peptide sequences
| Backbone | DNA constructs | Plasmids | Peptide sequences |
|---|---|---|---|
| pBAD-gIII B | HlyB–HlyD | pBAD-BD | – |
| pET-28a | TEVcs-6*His-HlyA | pET-THHly | – |
| His tag-TEVcs-6*His-HlyA | pET-HTHHly | HHHHHH | |
| T7 tag-TEVcs-6*His-HlyA | pET-TTHHly | MASMTGGQQMG | |
| S tag-TEVcs-6*His-HlyA | pET-STHHly | KETAAAKFERQHMDS | |
| Flag tag-TEVcs-6*His-HlyA | pET-FTHHly | DYKDDDDK | |
| Myc tag-TEVcs-6*His-HlyA | pET-MTHHly | EQKLISEEDL | |
| Cecropin A-TEVcs-6*His-HlyA | pET-CeATHHly |
KWKLFKKIEKVGQNIRDG IIKAGPAVAVVGQATQIAK |
|
| Melittin-TEVcs-6*His-HlyA | pET-MelTHHly |
GIGAVLKVLTTGLPALIS WIKRKRQQ |