TABLE 1.
PBMC cultured with or without UV-inactivated BHV-1a
| Phenotype | % of T-cell type inb:
|
Monoclonal antibody (isotype) | ||
|---|---|---|---|---|
| Fresh PBMC | 10-day-cultured PBMC
|
|||
| With inactivated BHV | Without inactivated BHV | |||
| CD2+ | 42.2 ± 3.6 | 83.8 ± 3.7 | 47.9 ± 2.2 | 16-1E10-B3 (IgG2a) |
| CD4+ | 24.6 ± 6.2c | 76.1 ± 3.3 | 25.6 ± 3.7 | IL-A12 (IgG2a) |
| CD8+ | 14.7 ± 20.7 | 14.8 ± 11.0 | 19.6 ± 10.5 | SBUT8 (IgG2a) |
| IgM+ | 5.0 ± 9.2 | 4.1 ± 0.6 | 1.2 ± 1.7 | 33 (IgG1) |
| WC1+d | 7.5 ± 27.2 | 11.4 ± 8.8 | 20.4 ± 17.4 | cc15 (IgG2a) |
a
PBMC were obtained from attenuated BHV-1 immunized cattle and cultured with UV-inactivated BHV-1 for 10 days.
b
The percentage of cells identified does not equal 100 because the CD2 molecule is present on CD4- and CD8-staining cells.
c
CD4+ T lymphocytes increased as the dominant cell population, ranging from 70 to 90% in five different experiments.
d
WC1 is a cell surface molecule that is expressed on bovine γδ T cells.