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. 2023 Mar 4;72(7):2233–2244. doi: 10.1007/s00262-023-03406-6

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© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 2 — Expression level of antioxidants in M2-TAMs. The frequency of TAMs and expression level of Nrf2 and HO-1 in TAMs were evaluated by flow cytometry. a The gating method to detect TAMs. b The summarized data of frequency of M1-, M1/M2-, and M2-TAMs infiltration in the normal mucosa site (n = 28) and tumor margin (n = 34). c Representative histograms of Nrf2 and HO-1 expressions in TAMs in the normal mucosa site (left) and tumor margin (right). d The summarized data of these expression levels in M1-, M1/M2-, and M2-TAMs in the normal mucosa site (left, n = 28) and tumor margin (right, n = 34). *p < 0.05, ****p < 0.0001