Fig. 4.

GC cell-derived exosomal miR-135b-5p was involved in the Vγ9Vδ2 T cell dysfunction. a The expression of miR-135b-5p in GES-1 cells, SGC7901 cells, MGC803 cells or MKN45 cells was analyzed by RT-qPCR. b The expression of miR-135b-5p in GES-1 cell-derived exosomes (GES-1 EXO) or MGC803 cell-derived exosomes (MGC803 EXO) was analyzed by RT-qPCR. c The expression of miR-135b-5p in Vγ9Vδ2 T cells co-treated with MGC803 exosome and Actinomycin D was analyzed by RT-qPCR. d The cell viability of Vγ9Vδ2 T cells treated with exosomes from MGC803 cells or MKN45 cells infected with LV-miR-135b-IN (EXO-miR-135b-IN) or LV-NC (EXO-NC-IN) was analyzed by CCK-8 assay. e, f The apoptosis rate e and the IFN-γ and TNF-α production f of Vγ9Vδ2 T cells treated with EXO-miR-135b-IN or EXO-NC-IN were detected by flow cytometry. Values are expressed as means ± SD. Data are representative of results from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001