Fig. 5.

SP1 is a functional target of miR-135b-5p. a The targets of miR-135b-5p were predicted by TargetScan, miRDB, miRWalk and starbase. b The Venn diagram of targets of miR-135b-5p and apoptosis or IFN-γ-associated with genes downloaded from GeneCards. c The binding position between miR-135b-5p and SP1 was predicted by TargetScan. d The luciferase activity of MGC803 cells co-transfected with the human SP1 wild-type (WT) 3ˈUTR or the SP1 mutant (MUT) 3ˈUTR and miR-135b-5p mimic (miR-135b-mimic), miR-135b-5p inhibitor (miR-135b-IN), control RNA mimic (NC-mimic) or control RNA inhibitor (NC-IN) was detected. e, f The protein expression of SP1 in MGC803 cells treated with NC-mimic, miR-135b-mimic, NC-IN, or miR-135b-IN was analyzed by western blotting. g The protein expression of SP1 in Vγ9Vδ2 T cells treated with exosomes from MGC803 cells infected with LV-miR-135b-IN (EXO-miR-135b-IN) or MGC803 cells infected with LV-NC (EXO-NC-IN) was analyzed by western blotting. Values are expressed as means ± SD. Data are representative of results from three independent experiments. **p < 0.01, ***p < 0.001