FIG. 3.

Detection of rFeLVs in buffy coat, bone marrow, and plasma samples from cat 5023 by nested PCR. Analyses of samples at 8, 14, and 32 weeks p.i. are displayed. While cell DNA was amplified for buffy coat and bone marrow specimens, plasma RNA was analyzed following the reverse transcription reaction. Plasma samples at earlier time points (2, 4, and 6 weeks p.i.) were determined to be negative and are not shown. The arrows mark the correct PCR product of ∼0.9 kb. Water and pFRA were used as negative controls in all experiments, while the FeLV-B/GA clone (pBHM-1) was used as positive control as indicated. The reverse transcription negative control with water at the reverse transcription step followed by nested PCR was also negative and is not shown.