Fig. 4.
HCF-specific cells from immunized mice produce high IL-5 and IFNɣ levels. After s.c. administration of LL/2 cells (100,000 cells/mouse) (day 0), C57BL/6 mice (n = 6) received a 300 μg dose of human HCF or PBS in alum on days 4, 7, and 11. At day 17 animals were sacrificed and spleens and DLN were collected. DLN cells (a) or splenocytes (b) (0.5–1 × 106/well) were cultured in presence or absence of a LL/2 lysate or HCF for three days at 37ºC. Levels of IL-5 and IFNγ were measured in cell supernatants by specific ELISA