Fig. 6.

NK1.1⁺ cells mediate HCF-induced protection. a After s.c. administration of LL/2 cells (day 0), C57BL/6 J mice received a 300 μg dose of human HCF or PBS in alum on days 4, 7, and 11. For NK1.1⁺ depletion, mice were injected i.p. with anti-NK1.1 antibody (300 μg/mouse) at days − 1,1, 3, 5, 7, 9, 12. Tumor growth was measured regularly using a caliper. Tumor volume (mm³) was calculated according to the following formula: 4/3 × pi x r1 x r2 x r3. b Survival of treated and control mice was followed for 60 days after tumor challenge. Mice were euthanized when subcutaneous tumors reached 20 mm or when mice shows signs of distress or discomfort. Statistically difference between PBS and HCF groups and PBS/α NK1.1⁺ and HCF groups