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PYK2 restored RB1CC1 inhibition of RCC cell lines. A. Western blot analysis of whole cell lysates of cells confirms DOX (1 µg/mL) induction and expression of RB1CC1 and PYK2 in A498i and ACHNi transfected with PYK2. B-C, The A498i and ACHNi cell viability measured by CCK-8 assay. D-E, A498i and ACHNi sphere-forming ability determined using sphere formation assay. F, A498i and ACHNi colony-forming ability determined using colony formation assay. *, & or # p < 0.05
