FIG. 3.

RNA triphosphatase activity. (A) Aliquots (3 μg of protein) of the 300 mM NaCl phosphocellulose eluate fraction of wild-type (WT) AcNPV phosphatase and the C119A mutant were electrophoresed through a 10% polyacrylamide gel containing 0.1% SDS. Polypeptides were visualized by staining with Coomassie blue dye. The positions and sizes (in kilodaltons) of marker proteins are indicated at the left. (B) RNA triphosphatase reaction mixtures containing 10 pmol of [γ-³²P]poly(A) and either wild-type (WT) or C119A protein as specified were incubated for 15 min at 30°C. The extent of ³²P_i release from poly(A) is plotted as function of input enzyme.