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Specific activity of the 9.5S and 2.5S forms of AcNPV RNA triphosphatase. Reaction mixtures containing 10 pmol of [γ-32P]poly(A) and glycerol gradient fraction 6 or 16 as specified were incubated for 15 min at 30°C. The extent of 32Pi release from poly(A) is plotted as function of input enzyme (expressed as femtomoles of the 23-kDa protomer).
