Fig. 1.

Effect of TIRS on T cell activation. The effects of various TIRS were evaluated with multiparameter intracellular cytokine staining after 8-h co-culture stimulation with autologous tumor cell lines, with a flow cytometry read-out. Black dots represent small molecules and empty dots represent antibodies. The background, TILs alone, was subtracted from the data and the data were normalized to tumor + TILs alone (visualized by the dotted line at 100%). The dot plots illustrate the effects of the drugs on CD8+ (a) and CD4+ (b) T cells. Tacrolimus, at 10 ng/ml and 100 ng/ml, and dexamethasone resulted in a significant reduction of CD8+ and CD4+ T-cell activation. Data are presented with median and tested for statistical significance using a Wilcoxon matched-pairs test. *p value = 0.01–0.05; MPA mycophenolate, TAC tacrolimus, DXM dexamethasone, VDZ vedolizumab, TOC tocilizumab, IFX infliximab