Fig. 2.

Effect of TIRS on T-cell-mediated tumor killing. The effect of various TIRS on T-cell-mediated tumor killing was measured via real-time tumor-killing assays on the xCELLigence platform and evaluated after a 24 h and b 60 h of co-culture of tumor, TILs, and drug. Small molecules were normalized to the control “tumor + TILs” and are represented as black dots, whereas all antibodies were normalized to the control “tumor + TILs + isotype” and are represented as empty dots. Controls are presented as a dotted line at 100%. a A significant reduction in T-cell-mediated killing was observed in the presence of dexamethasone after 24 h of co-culture. A trend of T-cell-mediated killing reduction is also evident with increasing doses of tacrolimus. b A significant reduction in T-cell-mediated killing was observed in the presence of mycophenolate, tacrolimus, and dexamethasone after 60 h of co-culture. Data are presented with median and tested for statistical significance using a Wilcoxon matched-pair test. p values: *0.01–0.05, **0.001–0.01; MPA mycophenolate, TAC tacrolimus, DXM dexamethasone, VDZ vedolizumab, TOC tocilizumab, IFX infliximab