Fig. 1.

PbAgo and BlAgo exhibit DNA-guided DNA cleavage activity at 37 °C. a Neighbor-joining phylogenetic tree analysis of PbAgo and BlAgo and characterized pAgos. The numbers at the nodes indicate the bootstrap values for neighbor-joining analysis of 1000 resampled data sets. The scale bar represents the evolutionary distance between species. The numbers in parentheses represent the sequence accession numbers in the NCBI database. b Synthetic DNA or RNA guides (red) and targets (blue). The predicted cleavage positions are indicated with a black triangle; black lines indicate the predicted 15 and 30 nt cleavage products. c Cleavage activity assays of PbAgo and BlAgo with FAM-labeled DNA or RNA targets