Fig. 2.

Tumor increased differentiation of T cells to regulatory phenotype both in vitro and in vivo. Expression of T_H cell subtype markers was assessed in spleen cells treated with TCM or isolated from TBM by real-time PCR. a Transcription factors specific to T_H1, T_H2, T_H17, Treg cells in TCM-treated spleen cells and b cytokines representative of T_H1, T_H2, T_H17, Treg in TCM-treated spleen cells. c Transcription factors specific to T_H1, T_H2, T_H17, Treg cells in TBM spleen cells and d cytokines specific to T_H1, T_H2, T_H17, Treg cells in TBM spleen cells. Data are represented as fold change in expression as compared to control. e Percentage of CD4⁺CD25⁺FOXP3⁺ cells obtained in TCM-treated spleen cells and those obtained from TCM from flow cytometric analysis along with the gating strategy. All data represented are mean ± SEM from three independent experiments. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 where treated is greater than control and ^#p ≤ 0.05, ^##p ≤ 0.01, ^###p ≤ 0.001 where treated is lesser than control