. 2022 Sep 14;9(1):98. doi: 10.1186/s40643-022-00590-8

Table 2.

96-well plate screening conditions of Capto Butyl ImpRes, Capto adhere ImpRes and Capto S ImpAct for both FabscFv-KiH and Fab₂scFv-KiH

	Buffer	pH	Salt
Capto Butyl ImpRes	Sodium citrate	4.0, 5.25, 6.5	20, 100, 200, 300, 400 mM Na-citrate
Capto adhere ImpRes	20 mM sodium citrate + 20 mM sodium phosphate	5.0, 5.75, 6.5	0, 50, 100, 150, 200, 250 mM NaCl
Capto S ImpAct	20 mM sodium citrate (pH 5.0–6.0) 20 mM sodium phosphate (pH 6.5–7.5)	5.0, 5.5, 6.0, 6.5, 7.0, 7.5	0, 50, 100 mM NaCl

Buffer

Salt

Capto Butyl ImpRes

Sodium citrate

4.0, 5.25, 6.5

20, 100, 200, 300, 400 mM Na-citrate

Capto adhere ImpRes

20 mM sodium citrate + 20 mM sodium phosphate

5.0, 5.75, 6.5

0, 50, 100, 150, 200, 250 mM NaCl

Capto S ImpAct

20 mM sodium citrate (pH 5.0–6.0)

20 mM sodium phosphate (pH 6.5–7.5)

5.0, 5.5, 6.0, 6.5, 7.0, 7.5

0, 50, 100 mM NaCl