Fig. 1.

Tissue processing schematic and characterization. (a) Schematic of tissue processing for characterization. Flow cytometry was conducted to evaluate cell composition following tissue dissociation and prior to 3D culture. Immunofluorescence and flow cytometry were conducted at different timepoints to evaluate 3D spheroid culture and drug treatment effects on cell populations. (b) Representative images of formalin-fixed paraffin embedded tissues. Pan-cytokeratin was used to determine the presence of epithelial cells within the tumor tissues. Additional immune-related markers selected to assess infiltration were PD-L1, PD-1, CD8, and CD11c. Representative images are from two independent experiments. Scale bar = 75 μm