Fig. 3.

T cell and DC populations within the 3D spheroid culture system. (a) Representative data and quantification of CD4+T cells (CD3+/CD4+). (b) Representative data and quantification of CD8+T cells (CD3+/CD8+). (c) Representative images verify the presence of CD8+cells as determined by immunofluorescence following 48 h in 3D spheroid culture. Scale bars = 75 μm. Two independent experiments and three independent experiments were conducted for Pre 3D and Post 3D, respectively. (d) The presence of DCs following 48 h in 3D spheroid culture was determined via immunofluorescence. Scale bars = 75 μm. Image inset shows a zoomed version of the same magnification. (e) Representative data for DCs defined by dual CD45+/CD11c+. The percent of dual CD45/CD11c events were determined for OVC45 and OVC33. Isotype controls (black events) were subtracted from marker values (OVC45 = blue events, OVC33 = green events). Expression of MHC-II and CD103 expression was determined for DC populations across the patient samples. Two independent experiments were conducted for OVC45, and three independent experiments were conducted for OVC33. Isotype controls were subtracted from marker values. Error bars reflect SD. Not significant = n.s., *p < 0.05, **p < 0.01, ***p < 0.005