Fig. 4.

Metastasis-specific differences underlying CD8⁺ T-cell heterogeneity along with immunosuppressive cells by multiplexed single-cell analysis. a Summary of the percent distribution by cell phenotype in 43 metastatic ccRCC samples (COHORT C). b–c Density of Foxp3⁺ Treg cells (b) and Foxp3⁺PD-1⁺ Treg cells (c) between 97 primary tumors and 43 metastases in ccRCC. d–f Metastasis-specific differences in cell phenotype distribution obtained from the bone (n = 16, d), lung (n = 8, e), and viscera (n = 9, f) tumor samples. g Density of CD8⁺ T-cells, CD8⁺CD39⁺ T-cells, CD8⁺CD39⁺PD-1⁺ T-cells, Foxp3⁺ Treg cells, Foxp3⁺PD-1⁺ Treg cells, and AE1/AE3⁺PD-L1⁺ cancer cells between bone/lung/visceral metastasis. The data are presented using violin and box plots. The line within the box is the median, the upper and lower ends of the box are the upper and lower quartiles, and the bars are the minimum and maximum values. p values were obtained from the two-tailed Mann–Whitney U test or Kruskal–Wallis H test