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. 2021 May 21;8(1):42. doi: 10.1186/s40643-021-00398-y

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© The Author(s) 2021

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Fig. 2 — Construction of mutant strains. a Genetic modifications to impair photorespiration and introduce the isoprene synthetic pathway. b Whole-cell PCR for the recombinant cassette harboring the ispS gene at the pta site; one primer was set 100 bp external to the recombinant cassette, which demonstrated the complete segregation of the Isps mutant. c Whole-cell PCR with specific primers demonstrating the integration of each gene into the chromosome of the recombinant strain MEP*-IspS. d Whole-cell PCR with specific primers for complete segregation of knocking out glcD1 and glcD2 gens in photorespiration impairing mutantsΔglcD1/D2, IspSΔglcD1/D2 and MEP*-IspSΔglcD1/D2