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. 2021 May 21;8(1):42. doi: 10.1186/s40643-021-00398-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Phenotype analysis of all strains. a Time-course of isoprene productions by the strains IspS, MEP*-IspS, IspSΔglcD1/D2 and MEP*-IspSΔglcD1/D2. b High-CO₂-requiring (HCR) phenotype analysis of the photorespiration-impaired strains ΔglcD1/D2, IspSΔglcD1/D2 and MEP*-IspSΔglcD1/D2. The strains were grown on BG11 plates with (HC) or without (LC) 50 mM NaHCO₃ under 100 μmol photons m⁻² s⁻¹ light condition. c Quantification of intracellular and extracellular glycolate in WT and all mutant strains grown on HC condition