Fig. 5.

OIT3 is a potential marker of M2 macrophages in GEO datasets and in vitro experiments. (a, b) Mrc1, Arg1, and OIT3 expression in M0, M1, and M2 macrophages in the validate dataset of GSE80815 array data (a) and GSE158094 RNA-seq data (b). (c–f) Expression of M1 and M2 markers as well as OIT3 in M0, M1 and M2 BMDM (c, d) and iBMDM (e, f). Data of a (n = 6), b (n = 3 in M0, n = 9 in M1 and M2), c (n = 3) and e (n = 6) are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data of d and f are normalized against the expression of Actin