Fig. 6.

OIT3 remodeled immunity and reprogrammed metabolism of macrophages. a Expression of OIT3 gene in iBMDM after transfected with mock or lentiviral vector of OIT3 and stimulated with PBS, LPS plus IFN, or IL-4 plus IL-13. Data are expressed as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ****p < 0.0001. b The protein expression of OIT3, mTOR, AMPKα in iBMDM after transfected with mock or lentiviral vector of OIT3 and stimulated with PBS, LPS plus IFN, or IL-4 plus IL-13 for 48 h, respectively. The orange values indicated the relative expression according to the density, the expression values were normalized against the expression of actin. c The transcription levels of molecules in promoting tumor (black), antiinflammatory (red), and proinflammatory (green) cytokines or chemokines, enzymes of glycolysis (blue), and fatty acid metabolism (orange) in M0, M1, and M2 macrophages after transfected with mock or lentiviral vector (LV) of OIT3