Fig. 3.

BAM-SiPc-PDT induced the translocation of CRT through a classical pathway. CT26 cells were first treated with 4 nM BAM-SiPc before the following experiments. a The PDT-treated cells were incubated for the indicated times. Cell lysates were used to detect the phosphorylation of PERK and eIF-2α through Western blotting. b, c The PDT-treated cells were incubated with and without the various pathway inhibitors for 4 h. b The cells were stained with PI and anti-CRT antibody and subjected to flow cytometric analysis. The population stained represents cell population of PI negative and CRT positive. c The cell membrane proteins were isolated for Western blotting to probe for CRT. d CT26 cells were transfected with corresponding siRNA to restrain PERK and caspase 8 expressions. The suppressive effects were shown in Western blot analyses. e After transfection, PDT was performed 48 h later. Having been stained with PI and anti-CRT antibody, cells were subjected to flow cytometric analysis. Data shown are means ± SEM or representative results of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001