Fig. 5.

ALDH peptide(s)-DC vaccine induced humoral immune responses and CD3⁺ TILs a: ALDH peptide(s)-DC vaccine primed mice to produce plasma antibody which bound to ALDH^high D5 CSCs. Flow cytometry scatter plots demonstrated the representative binding rate of plasma IgG to ALDH^high D5 CSCs, and the histogram graph showed the mean ± standard error of the plasma binding rate to ALDH^high D5 CSCs. This experiment was repeated three times. b: ALDH peptide(s)-DC vaccine-primed plasma antibody lysed ALDH^high D5 CSCs via CDC. ALDH^low D5 non-CSCs were used as target control. This experiment was repeated twice. c: Flow cytometry showing the percentage of ALDH^high CSCs in residual D5 tumors post-treatment as indicated. d: ALDH peptide-DC vaccination treatments induced CD3⁺ TILs. Representative IHC results showing CD3⁺ TILs in residual tumors harvested from the treated hosts. Tumor cells and background were stained with red and amaranth, respectively. The experiment was repeated twice. e: Bar graph comparing the CD3⁺ TILs induced by different treatments as indicated